The Mhor Marathon starts from McLaren High School, Callander. After leaving the school grounds the route takes runners off the road and on to a trail path through Coilhallon Wood. Emerging out of the wood there is a short section on then off road before joining Sustrans Route 7 at Kilmahog. The route then follows Garbh Uisge (River Leny) north towards Strathyre along the length of Loch Lubnaig. It offers breathtaking views of both the Loch and surrounding mountains including Ben Vorlich, Stuc a' Chroin and Ben Ledi.

Arriving into Strathyre after 10.5 miles runners head towards Stronvar and Balquhidder parallel to the River Balvaig. Once in Balquhidder the route turns right to pass the burial site of Rob Roy MacGregor at Balquhidder Kirkyard and continues along the road to Mhor 84 Motel. Just before reaching the Motel runners will turn right onto Route 7 and run back to Strathyre Village to complete the loop section of the course. After retracing their steps back to Stronvar, the route turns left and sends runners down the South Side of Loch Voil towards Tuarach and Loch Doine. The view down the length of Balquhidder Glen is spectacular and as runners follow the undulating track to the finish they will see the imposing Ben More in the distance. The final mile includes a descent to cross the river between the Lochs and then a few hundred metres through the fields to the finish at Monachyle Mhor Hotel. 

Throughout the route runners will pass Loch Lubnaig, Loch Voil and Loch Doine, follow the path of the River Leny and River Balvaig and be surrounded at all times by the breathtaking mountainous landscape of the Trossachs. The Mhor Marathon undoubtedly showcases some of the most spectacular scenery Scotland has to offer, not to mention the award-winning Mhor hospitality on offer at the finish line.